Estudio toxicogenético de un extracto fluido de ocimun basilicum l. (albahaca blanca)

Abstract

Summary The results obtained on evaluating the toxicogenetic potential of a fluid extract with an ethanol menstruum 70 % of Ocimun basilicum L. in 3 systems of short-term tests are presented. 2 in vitro models were used: the Salmonella/microsome system (Ames) and that of mitotic segregation with the Aspergillus nidulans D-30 fungus. Another in vivo model that uses the micronucleus induction test in mouse bone marrow was also included. A positive response was observed in the Amesi test with the strains of Salmonella typhimurium TA-98 with metabolic activation in the range of concentrations of 1 500 and 5 000 μg/plate and TA-1535 without metabolic activation in the range of concentrations of 50, 150, 500 and 1 500 μg/plate. Concentrations from 0,005 to 1,00 mg of total solids/mL were evaluated in the mitotic segregation test. No significant concentration-depending increases were found in the Frequency of Segregating Sectors by Colonies (FSC). In the micronucleus induction test, doses of 500, 1 000 and 2 000 mg/kg of body weight were assayed. The results showed that the extract of Ocimun basilicum L. proved to be mutagenic in the Salmonella/microsome system and moderately cytotoxic in the mitototic segregation test. No genetic damage was detected in this assay. Subject headings: PLANTS, MEDICINAL/genetic; PLANTS, MEDICINAL/toxicity; PLANT EXTRACTS; ASPERGILLUS NIDULANS/genetic; SALMONELLA TYPHIMURIUM; MICE; MEDICINE, HERBAL; ALBAHACA/genetic; ALBAHACA/ toxicity; MUTAGENICITY TESTS; MICRONUCLEUS TESTS.