Revisión de la Caracterización de Aeromonas spp. Y su Importancia clínica
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Rev. Bol. Quim
Abstract
Los recientes avances en Microbiología y la aplicación de la Biología Molecular, han logrado un mejoramiento, complementario para las técnicas clásicas de identificación bacteriana, también esto, ha permitido el descubrimiento o reagrupamiento de géneros y especies bacterianas, las cuales no habían logrado ser identificadas correctamente, por métodos bioquímicos y fenotípicos, ya que estos, presentan algunas limitaciones como falsos positivos o baja especificidad. Dentro de las herramientas moleculares empleadas para la identificación del género Aeromonas, se han empleado la secuenciación a partir de gen 16S de rRNA, genes de mantenimiento como rpoD o gyrB, los cuales poseen mayor poder de discriminación que el gen 16S de rRNA, la técnica de RFLP-PCR y más recientemente se ha incorporado el uso de la técnica de Espectrofotometría de Masas por Matriz Asistida por laser Desorción-Ionización de Tiempo de Vuelo (MALDI-TOF MS), la cual es capaz de identificar especies de Aeromonas de forma precisa y rápidamente. Lo cual es de gran importancia, debido al incremento de evidencias que indican que estas especies se encuentran ampliamente distribuidas en el ambiente y que pueden causar una variedad de infecciones en animales y en el humano.
Advances in Microbiology and applications of Molecular Biology, has been achieved an improvement, complementary to the classical techniques of bacterial identification, this also allowed the discovery or regrouping of bacterial genera and species, which had failed to be identified correctly, by biochemical and phenotypic methods, since these have some limitations such as false positives or low specificity. Among the molecular tools used to identify the Aeromonas genus, we have used sequencing from 16S rRNA gene, maintenance genes such as rpoD or gyrB, which have a greater discrimination power than the 16S rRNA gene, the technique of RFLP-PCR and more recently the technique of Mass Spectrometry has been increased by Laser Assisted Matrix Desorption-Ionization of Flight Time (MALDI-TOF MS) which is able to identify species of Aeromonas quickly and accurately. This is important because of the increasing evidence that these species are widely distributed in the environment and can cause a variety of infections in animals and humans.
Advances in Microbiology and applications of Molecular Biology, has been achieved an improvement, complementary to the classical techniques of bacterial identification, this also allowed the discovery or regrouping of bacterial genera and species, which had failed to be identified correctly, by biochemical and phenotypic methods, since these have some limitations such as false positives or low specificity. Among the molecular tools used to identify the Aeromonas genus, we have used sequencing from 16S rRNA gene, maintenance genes such as rpoD or gyrB, which have a greater discrimination power than the 16S rRNA gene, the technique of RFLP-PCR and more recently the technique of Mass Spectrometry has been increased by Laser Assisted Matrix Desorption-Ionization of Flight Time (MALDI-TOF MS) which is able to identify species of Aeromonas quickly and accurately. This is important because of the increasing evidence that these species are widely distributed in the environment and can cause a variety of infections in animals and humans.
Description
Vol. 34, No. 5