Evaluación de bacterias endófitas promotoras de crecimiento en el cultivo de quinua
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J. Selva Andina Biosph.
Abstract
En la presente investigación se evaluó la interacción simple y combinada de bacterias endófitas, para desarrollar nuevas formulaciones de bioinoculantes y así generar alternativas viables para los productores andinos sobre las variables agronómicas del cultivo de quinua. Se formularon bioinoculantes con tres cepas, solas y combinadas de Bacillus sp., junto con un sustrato inerte para obtener un producto en polvo, así posteriormente compararlo con el producto comercial (TRICOBAL), como testigo positivo, más el testigo negativo (suelo sin inoculante), bajo condiciones in vitro e invernadero. Se identificaron como sobresalientes a los siguientes tratamientos, en cuanto a la altura de planta (104.14 cm), altura de panoja (34.32 cm), peso de raíces (5 g) y rendimiento en grano: Bacillus pumilus (Bp), la interacción B. pumilus y P. polymyxa (Bp + Bv54) y la combinación de B. pumilus, B. suhtilis y P. polymyxa (Bp + Bs + Bv54), señalando que éstas cepas nativas promueven el crecimiento de las plantas de quinua significativamente superior al producto comercial TRICOBAL. Complementariamente, en condiciones in vitro se determinó que las tres cepas de Bacillus utilizadas en el estudio pueden coexistir.
The present research aims to evalúate the interaction of simple and combined endophytic bacteria, to develop new bioinoculants formulations, over the agronomic variables of the quinoa cultivation, to genérate viable alternatives for the Andean farmers. A powder product was obtained by mixing a bioinoculants formulation developed with three single and combined strains of Bacillus sp. and an inert substrate, to be compared in a greenhouse/in vitro environment with a commercial product (TRICOBAL) as a positive control group and normal soil (without inoculants) as a negative control group. The following treatments were identified: Bacillus pumilus (Bp), interaction between Bacillus pumilus and Paenihacillus polymyxa (Bp + Bv54) and the combination of Bacillus pumilus, Bacillus suhtilis and Paenihacillus polymyxa (Bp + Bs + Bv54) with prominent results on: plant height (104.14 cm), panicle height (34.32 cm), roots weight (5 grs.) and grain performance; demonstrating that these native strains significantly promote the growth of quinoa plants in relation to the commercial product (TRICOBAL). In addition, it was determined that in an in vitro environment the three used strains of Bacillus can coexist.
The present research aims to evalúate the interaction of simple and combined endophytic bacteria, to develop new bioinoculants formulations, over the agronomic variables of the quinoa cultivation, to genérate viable alternatives for the Andean farmers. A powder product was obtained by mixing a bioinoculants formulation developed with three single and combined strains of Bacillus sp. and an inert substrate, to be compared in a greenhouse/in vitro environment with a commercial product (TRICOBAL) as a positive control group and normal soil (without inoculants) as a negative control group. The following treatments were identified: Bacillus pumilus (Bp), interaction between Bacillus pumilus and Paenihacillus polymyxa (Bp + Bv54) and the combination of Bacillus pumilus, Bacillus suhtilis and Paenihacillus polymyxa (Bp + Bs + Bv54) with prominent results on: plant height (104.14 cm), panicle height (34.32 cm), roots weight (5 grs.) and grain performance; demonstrating that these native strains significantly promote the growth of quinoa plants in relation to the commercial product (TRICOBAL). In addition, it was determined that in an in vitro environment the three used strains of Bacillus can coexist.
Description
Vol. 7, No. 2