Efecto de seis sustratos energéticos sobre el tiempo de la viabilidad espermática del semen de Alpaca (Vicugna pacos L.)
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J.Selva Andina Anim. Sci.
Abstract
La investigación se realizó en el Laboratorio de Biotecnología Reproductiva de la UAC Tiahuanacu, provincia Ingavi del departamento de La Paz, dependiente de la Universidad Católica Boliviana San Pablo, cuyo objetivo fue evaluar el efecto de seis sustratos energéticos sobre el tiempo de viabilidad espermática del semen de alpaca (Vicugna pacos L.). El semen colectado fue diluido y conservado en seis diferentes sustratos energéticos (TCG, TCAP, TCF, TG, TAP y TF), una Temperatura de 35 ºC, evaluando cada 2 h, empleando para su análisis el Diseño Completamente al Azar (P<0.05). Obteniendo un promedio de volumen de semen 1.5±0.7 mL, filancia 5.1±1.5, pH 7.2±0.5, motilidad y vitalidad de 89.0 y 95.0 % y concentración 82.0x10(6) Epz/mL. El sustrato energético (SE) TCG logró mantener una motilidad de 61.0 y 66.0 % de vitalidad conservado durante 8 horas, en tal sentido se recomienda utilizar el SE TCG, mismo que mantuvo por mayor tiempo (horas) a los espermatozoides viables para la IAF.
The research was carried out in the Laboratory of Reproductive Biotechnology of the UAC Tiahuanacu, Ingavi province of the Department of La Paz, dependent of the Bolivian Catholic University, whose objective was to evaluate the effect of six energetic substrates on the sperm viability time of the alpaca semen (Vicugna pacos L.). The collected semen was diluted and stored in six different energy substrates (TCG, TCAP, TCF, TG, TAP and TF), a T ° of 35 ºC, evaluating every 2 hours, using the Completely Random Design (P < 0.05). Obtaining a mean volume of semen 1.5 ± 0.7 mL, filament 5.1 ± 1.5, pH 7.2 ± 0.5, motility and vitality of 89.0 and 95.0% and concentration 82.0x106 Epz / mL. The energetic substrate (SE) TCG managed to maintain a motility of 61.0 and 66.0% of vitality preserved for 8 hours, in that sense it is recommended to use the SE TCG, which maintained for a longer time (hours) the viable spermatozoa for LAI.
The research was carried out in the Laboratory of Reproductive Biotechnology of the UAC Tiahuanacu, Ingavi province of the Department of La Paz, dependent of the Bolivian Catholic University, whose objective was to evaluate the effect of six energetic substrates on the sperm viability time of the alpaca semen (Vicugna pacos L.). The collected semen was diluted and stored in six different energy substrates (TCG, TCAP, TCF, TG, TAP and TF), a T ° of 35 ºC, evaluating every 2 hours, using the Completely Random Design (P < 0.05). Obtaining a mean volume of semen 1.5 ± 0.7 mL, filament 5.1 ± 1.5, pH 7.2 ± 0.5, motility and vitality of 89.0 and 95.0% and concentration 82.0x106 Epz / mL. The energetic substrate (SE) TCG managed to maintain a motility of 61.0 and 66.0% of vitality preserved for 8 hours, in that sense it is recommended to use the SE TCG, which maintained for a longer time (hours) the viable spermatozoa for LAI.
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Vol. 4, No. 1