Efecto de dilutores y tiempos de equilibrio en la criopreservación de semen ovino (Ovis aries)
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Abstract
La crianza y producción de ovinos en Bolivia es de importancia social y económica, la criopreservación y la inseminación artificial son técnicas reproductivas para el progreso genético. Sin embargo, la inseminación con semen congelado no es satisfactorio por la baja tasa de fertilidad, que puede ser prevenido con el uso de un dilutor adecuado. El objetivo de la investigación es evaluar y comparar el efecto de tres dilutores seminales en dos tiempos de equilibrio sobre la motilidad de espermatozoides ovinos para la criopreservación. El trabajo se desarrolló en el laboratorio de crioconservación de la Estación Experimental de Choquenaíra, Universidad Mayor de San Andrés, el material biológico fue tres ovinos machos en edades reproductivas de 1.7, 2.7 y 4.5 años de la raza Corriedale, los dilutores utilizados fueron T-G-Y, TRILADYL y C-Y en los tiempos de equilibrio de cuatro y ocho horas. Se evaluó el efecto de la edad sobre las características macroscópicas, motilidad masal y espermática en el semen diluido y congelado, motilidad espermática al post-descongelamiento para dilutores y para tiempos de equilibrio. La motilidad masal fue de 55.55%, 38.89% y 5.55% para las escalas 5, 4 y 3 de Herman Swanson. Las edades no influenciaron en la motilidad individual, pero influyeron en el porcentaje de espermatozoides muertos con el 12.67%, 10.00% y 7.50% y porcentaje de espermatozoides anormales con 15.80%, 13.00% y 9.70% para las edades de 1.7, 4.5 y 2.7 años. La concentración de espermatozoides fue de 3.14x10(9) ml-¹, 3.12x10(9) ml-¹ y 2.86x10(9) ml-¹ y la motilidad espermática fue de 66.50, 66.33 y 64.50% para 2.7, 4.5 y 1.7 años, los dilutores mostraron una motilidad del 67.67% T-G-Y, 65.17% C-Y y 64.50% TRILADYL con influencia del 68.22% en cuatro horas y 63.33% en ocho horas de tiempos de equilibrio. La edad del ovino para obtener mejor calidad del semen es de 4.5 años y el dilutor T-G-Y es la mejor opción para obtener mayor motilidad espermática y prevenir daños en los espermatozoides durante su proceso de criopreservación, lográndose mayores tasas de fertilidad al momento de la inseminación artificial.
The breeding and production of sheep in Bolivia is important in social and economic aspects, cryopreservation and artificial insemination are reproductive techniques forgenetic progress. However, insemination with frozen semen is not satisfactory because of the low fertility rate, which can be prevented with the use of an adequate dilutor. The objective of the research is to evaluate and compare the effect of three seminal diluters in two equilibrium times on ovum sperm motility for cryopreservation. The work was developed in the cryopreservation laboratory of the Experimental Station of Choquenaíra, of the Major San Andrés University, the biological material was three male sheep in reproductive ages of 1.7, 2.7 and 4.5 years of the Corriedale breed. Used diluents were TGY, TRILADYL and CY at equilibrium times of four and eight hours. The effect of age on macroscopic characteristics, mass and sperm motility in the diluted and frozen semen, post-thaw sperm motility for diluents and for equilibrium times was evaluated. The mass motility was 55.55%, 38.89% and 5.55% for Scales 5, 4 and 3 of Herman Swanson. The ages did not influence the individual motility, butthey influenced the percentage of dead sperm with 12.67%, 10.00% and 7.50% and percentage of abnormal sperm with 15.80%, 13.00% and 9.70% for the ages of 1.7, 4.5 and 2.7 years. The sperm concentration was 3.14x109 ml-¹, 3.12x109 ml-¹ and 2.86x109 ml-¹ and the sperm motility was 66.50%, 66.33% and 64.50% for 2.7, 4.5 and 1.7 years, the diluters showed a motility of 67.67 % TGY, 65.17% CY and 64.50% TRILADYL with influence of 68.22% in four hours and 63.33% in eight hours of equilibrium times. The age of the sheep to obtain better semen quality is 4.5 years and the TGY dilutor is the best option to obtain greater sperm motility and prevent sperm damage during its cryopreservation process, achieving higher fertility rates at the time of artificial insemination.
The breeding and production of sheep in Bolivia is important in social and economic aspects, cryopreservation and artificial insemination are reproductive techniques forgenetic progress. However, insemination with frozen semen is not satisfactory because of the low fertility rate, which can be prevented with the use of an adequate dilutor. The objective of the research is to evaluate and compare the effect of three seminal diluters in two equilibrium times on ovum sperm motility for cryopreservation. The work was developed in the cryopreservation laboratory of the Experimental Station of Choquenaíra, of the Major San Andrés University, the biological material was three male sheep in reproductive ages of 1.7, 2.7 and 4.5 years of the Corriedale breed. Used diluents were TGY, TRILADYL and CY at equilibrium times of four and eight hours. The effect of age on macroscopic characteristics, mass and sperm motility in the diluted and frozen semen, post-thaw sperm motility for diluents and for equilibrium times was evaluated. The mass motility was 55.55%, 38.89% and 5.55% for Scales 5, 4 and 3 of Herman Swanson. The ages did not influence the individual motility, butthey influenced the percentage of dead sperm with 12.67%, 10.00% and 7.50% and percentage of abnormal sperm with 15.80%, 13.00% and 9.70% for the ages of 1.7, 4.5 and 2.7 years. The sperm concentration was 3.14x109 ml-¹, 3.12x109 ml-¹ and 2.86x109 ml-¹ and the sperm motility was 66.50%, 66.33% and 64.50% for 2.7, 4.5 and 1.7 years, the diluters showed a motility of 67.67 % TGY, 65.17% CY and 64.50% TRILADYL with influence of 68.22% in four hours and 63.33% in eight hours of equilibrium times. The age of the sheep to obtain better semen quality is 4.5 years and the TGY dilutor is the best option to obtain greater sperm motility and prevent sperm damage during its cryopreservation process, achieving higher fertility rates at the time of artificial insemination.
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Vol. 4, No. 2