Variabilidad del microbioma oral en biopelícula supragingival de niños con caries dental: estudio de cohorte
Date
Journal Title
Journal ISSN
Volume Title
Publisher
Gac Med Bol
Abstract
Objetivos: comparar la variabilidad del microbioma oral asociado a caries en una cohorte de niños de 5 - 9 años con seguimiento a 6 meses de un sector de la ciudad de Cartagena. Métodos: diseño de estudio observacional y analítico de una cohorte retrospectiva de 69 registros con seguimiento a 6 meses de niños de 5-9 años de dos instituciones educativas de Cartagena. Se evaluó la presencia de caries, severidad y variabilidad de Streptococcus spp por la técnica de secuenciación de próxima generación (HOMINGS). El análisis de los datos se llevó a cabo utilizando el software SPSS v29, con una significancia estadística considerada en valor menor p< 0,05. Se obtuvo la aprobación de aval ético por parte de la institución ejecutora. Resultados: se encontraron 26 registros en total, de los cuales el 65,4% desarrollaron caries dental, siendo el 50% de estas en estadio inicial. Durante el estudio, se observaron variaciones en la abundancia relativa de Streptococcus spp, especialmente en las sondas 4 y 1, así como en las especies S. sanguinisy S. mutans a lo largo del tiempo. Se identificó que niveles más bajos de Streptococcus sonda 4 mostraron una ligera tendencia hacia un mayor riesgo de desarrollar caries dental (OR: 1,07; IC95%: 0,150-7,54; p=0,978); sin embargo, estas diferencias no fueron significativas. Conclusiones: las dinámicas en el tiempo mostraron una variabilidad del microbioma oral de Streptococcus spp. Es necesario evaluar las interacciones con otros factores de riesgos que permitan identificar sus relaciones el inicio, desarrollo y progresión de la enfermedad.
Objectives: to compare the variability of the oral microbiome associated with caries in a cohort of children aged 5-9 years with a 6-month follow-up from a sector of the city of Cartagena. Methods: observational and analytical study design of a retrospective cohort of 69 records with a 6-month follow-up of children aged 5-9 years from two educational institutions in Cartagena. The presence of caries, severity and variability of Streptococcus spp was evaluated by the next generation sequencing technique (HOMINGS). Data analysis was performed using SPSS v29 software, with a value less than 0.05 considered significant. Ethical approval was obtained from the implementing institution. Results: a total of 26 records were found, of which 65.4% developed dental caries, 50% of which were in the initial stage. During the study, variations in the relative abundance of Streptococcus spp were observed, especially in probes 4 and 1, as well as in the species S. sanguinis and S. mutans over time. It was identified that lower levels of Streptococcus probe 4 showed a slight tendency towards a higher risk of developing dental caries (OR: 1.07; 95% CI: 0.150-7.54; p=0.978); however, these differences were not significant. Conclusions: the dynamics over time showed a variability of the oral microbiome of Streptococcus spp. It is necessary to evaluate the interactions with other risk factors that allow identifying their relationships with the onset, development and progression of the disease.
Objectives: to compare the variability of the oral microbiome associated with caries in a cohort of children aged 5-9 years with a 6-month follow-up from a sector of the city of Cartagena. Methods: observational and analytical study design of a retrospective cohort of 69 records with a 6-month follow-up of children aged 5-9 years from two educational institutions in Cartagena. The presence of caries, severity and variability of Streptococcus spp was evaluated by the next generation sequencing technique (HOMINGS). Data analysis was performed using SPSS v29 software, with a value less than 0.05 considered significant. Ethical approval was obtained from the implementing institution. Results: a total of 26 records were found, of which 65.4% developed dental caries, 50% of which were in the initial stage. During the study, variations in the relative abundance of Streptococcus spp were observed, especially in probes 4 and 1, as well as in the species S. sanguinis and S. mutans over time. It was identified that lower levels of Streptococcus probe 4 showed a slight tendency towards a higher risk of developing dental caries (OR: 1.07; 95% CI: 0.150-7.54; p=0.978); however, these differences were not significant. Conclusions: the dynamics over time showed a variability of the oral microbiome of Streptococcus spp. It is necessary to evaluate the interactions with other risk factors that allow identifying their relationships with the onset, development and progression of the disease.
Description
Vol. 48, No. 1